a-Glucosidase and N-Acetyl-p-o-glucosaminidase Isoenzymes in Serum
نویسندگان
چکیده
Usingdifferent conditions for incubation and fluorometry with 4-methylumbelliferylglycosides as substrates, we demonstratedthe presence ofacida-glucosidase, “renal” a-glucosidase, N-acetyl--D-glucosaminidase A, and N-acetyl-f3-o-glucosaminidase B in freshly drawn normal human serum. The acid a-glucosidase enzymatic activity was determined at pH 4.0 in 0.1 mol/L Tris reagent, whereasthe renal isoenzymeactivitywas determinedat pH 5.6 in presence of 0.05 mol/L turanose reagent. N-Acetyl.13-D-glucosaminidases A andBweredetermined bytheirdifferentbehaviorson heating.Thecorresponding referenceintervalsforeach enzymewerecalculatedfrom resultsfor40 controls:acida-glucosidase(0.024 ± 0.010 U/L), renala-glucosidase(0.035 ± 0.012 U/L), N-acetyl-D-glucosaminidase A (10.2 ± 2.9 U/L), and N-acetyl-o-glucosaminidase B (4.4 ± 2.1 U/L).
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